Processes, Tasks and Procedures (Cyril King)

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      While participating in the CRISP (Center for Research on Interface Structures and Phenomena) and using the IMP-MS to characterize element composition, find the number of particles, find the size of nano-particles, distribute size of nano-particles and analyze single nano-particles I have learned a collection of procedures and processes for handling the instrument. For instance, when diluting a solution to a lower concentration, it could be easily analyzed in the ICP-MS. The method for diluting a solution involves:
"20,000ppb"

1) Use micro-pipette to take  0.1mL of stock solution (Iron Sucrose) and place it in a 100ml volumetric flask.

2) Use micro-pipette to take 99.9mL of deionized water and mix it with the 0.1mL of stock solution in the volumetric flask.

3) Pour the 99.9mL of deionized water and 0.1mL of stock solution mix into a vile

4) label the vile "20,000ppb".

5) Place "20,000ppb" in sonicator until grabbing it to be ready for the next dilution.

Dilute "20,000ppb" to get "200ppb"

1) Place 1mL of deionized water in a 25mL volumetric flask using a pipette.

2) Use micro-pipette to get .25mL of "20,000ppb" (Stock Soulution + 99.9mL of Di) and mix it with the 1mL of deionized water in a 25mL volumetric flask.

3) Fill the 25mL volumetric flask with deionized water.

4) Pour the .25ml of "20,000ppb" (Stock Soulution + 99.9mL of Di) and 24.75mL of deionized water into a vile.

5) label the vile "200ppb".

6) Place "200ppb" in sonicator until grabbing it to be ready for the next dilution.

Dilute "20,000ppb" to get "100ppb"

1) Place 1mL of deionized water in a 25mL volumetric flask using a pipette.

2) Use micro-pippette to get .125mL of "20,000ppb" (Stock Soulution + 99.9mL of Di) and mix it with the 1mL of deionized water in a 25mL volumetric flask.

3) Fill the 25mL volumetric flask with deionized water.

4) Pour the .125ml of "20,000ppb" (Stock Soulution + 99.9mL of Di) and 24.875mL of deionized water into a vile.

5) label the vile "100ppb".

6) Place "100ppb" in sonicator until grabbing it to be ready for the next dilution.

 Dilute "200ppb" to get "8ppb"

1) Place 1mL of deionized water in a 25mL volumetric flask using a pipette.

2) Use micro-pipette to get 1mL of "200ppb" and mix it with the 1mL of deionized water in a 25mL volumetric flask.

3) Fill the 25mL volumetric flask with deionized water.

4) Pour the 1ml of "200ppb" and 24mL of deionized water into a vile.

5) label the vile "8ppb".

6) Place "8ppb" in sonicator until grabbing it to be ready for the next dilution.
  Dilute "8ppb" to get "0.32ppb"

1) Place 1mL of deionized water in a 25mL volumetric flask using a pipette.

2) Use micro-pipette to get 1mL of "8ppb" and mix it with the 1mL of deionized water in a 25mL volumetric flask.

3) Fill the 25mL volumetric flask with deionized water.

4) Pour the 1ml of "8ppb" and 24mL of deionized water into a vile.

5) label the vile "0.32ppb".

6) Place "0.32ppb" in sonicator until grabbing it to be ready for the next dilution.

The Daily Process for the ICP-MS involves (set up routine): 

1) Make sure the coolant is on.

2) Turn on Computer and set up the Syngistx program.

3) Turn on the ICP-MS and start the plasma and wait 5-10 minutes.

4) While plasma is running to make sure the pump is drawing out solution steadily and the tubes are set up.

5) Go through the daily performance check under "Smartune" in Syngistx.

6) Once the performance check passes move onto the method.






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