Process, Tasks, and Procedure (Daniela)
I spend most of my time in the lab observing the different projects going on and taking pictures on the different microscopes. I've gotten better at imaging cellulose fibers and nanotubes, although taking measurements and collecting data seems to be the most difficult part. There is a lot of data analysis involved in the projects here at CRISP.
For my project, high surface and low surface nanotubes must be prepared for inspection under the microscope and two different concentrations of the nanotubes will be used. First is the threshold concentration (21mM), this is the concentration where you can begin to observe the formation of nanotubes; the second concentration (270 mM) is the medicinal concentration. In order to form the nanotubes, a powder must be combined with water (specific measurements coming soon). The solution must be stored overnight and then it will be placed on a grid for inspection under transmission electron microscope (TEM).
Most of the process involves the preparation of the nanotubes, as of now the 270 mM sample is troublesome to prepare because of its viscosity, making it difficult to stay on the grid.
When looking for nanotubes under the microscope, careful attention to detail is necessary, you have to be sure that what you're looking at isn't dust. The TEM has a camera installed so pictures can be taken of the nanotubes.
In order to compare different types of nanotubes, measurements of the length and width of the nanotubes must be taken. Using ImageJ software, these measurements can be made much more easily.
I'm not sure if we'll have time, but there us a chance that the nanotubes will be tested using the spectrometer.
For my project, high surface and low surface nanotubes must be prepared for inspection under the microscope and two different concentrations of the nanotubes will be used. First is the threshold concentration (21mM), this is the concentration where you can begin to observe the formation of nanotubes; the second concentration (270 mM) is the medicinal concentration. In order to form the nanotubes, a powder must be combined with water (specific measurements coming soon). The solution must be stored overnight and then it will be placed on a grid for inspection under transmission electron microscope (TEM).
Most of the process involves the preparation of the nanotubes, as of now the 270 mM sample is troublesome to prepare because of its viscosity, making it difficult to stay on the grid.
When looking for nanotubes under the microscope, careful attention to detail is necessary, you have to be sure that what you're looking at isn't dust. The TEM has a camera installed so pictures can be taken of the nanotubes.
In order to compare different types of nanotubes, measurements of the length and width of the nanotubes must be taken. Using ImageJ software, these measurements can be made much more easily.
I'm not sure if we'll have time, but there us a chance that the nanotubes will be tested using the spectrometer.
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